Fed-batch Fermentation

Feed batch fermentation, also known as semi continuous fermentation or semi continuous culture, refers to the cultivation method of intermittently or continuously adding fresh culture medium during the batch fermentation process. Compared to traditional batch fermentation, its advantage lies in maintaining a very low substrate concentration in the fermentation system. The advantages of low substrate concentration are: ① it can eliminate the inhibitory effect of rapid utilization of carbon sources and maintain an appropriate bacterial concentration, so as not to exacerbate the contradiction of oxygen supply; ② Avoid the accumulation of toxic metabolites in the culture medium.
Feed batch fermentation is widely used in the production of antibiotics, amino acids, enzyme preparations, nucleotides, organic acids, and polymers.