Closed Continuous Fermentation

In a closed continuous fermentation system, a certain method is used to keep cells in the bioreactor and continuously increase their number. Under these conditions, certain limiting factors change in the bioreactor, leading to the majority of cell death. Therefore, in this system, it is impossible to maintain a stable state. Closed continuous fermentation can be modified using open continuous fermentation equipment, as long as some bacterial bodies are used for re circulation. Another method is to use spacers or fillers placed inside the equipment to allow the bacteria to grow on top, and the fermentation broth flows out without or with very few cells.
Dialysis membrane continuous fermentation is a new method. It uses an organic membrane with micropores to separate the fermentation equipment. This membrane can only pass through the fermentation products, but not through bacterial cells. In this way, the culture medium is continuously added to the interval with bacterial bodies in the fermentation equipment, and the metabolic products of the microorganisms continuously flow out from another interval through the dialysis membrane. In some fermentation processes, when the accumulation of metabolites in the fermentation broth reaches a certain level, it will be inhibited from continuing to accumulate. However, the use of dialysis membrane fermentation method can continuously dialysis out the metabolites, leaving little residue in the fermentation broth, thus improving product yield.